The hemin-induced accumulation of hemoglobin was inhibited in ERK1-KR overexpressing cells and was enhanced in the wild-type ERK1 transfectants. There was no activation of JNK or p38 protein kinases. During this time, fetal hemoglobin synthesis also increases from 0.8 to 10 pg/cell. The ERK1 and 2 activity increased within 2 h post hemin treatment and remained elevated for 24-48 h. We examined the activation of MAP kinases during hemin-induced differentiation.
In the presence of exogenously added hemin, K562 cells differentiate into erythroblasts, as indicated by the production of large amounts of fetal hemoglobin. Incubation with the MEK1 inhibitor UO126 inhibited cell growth and induced apoptosis in K562 cells in a dose-dependent manner as well. Overexpression of ERK1-KR inhibited cell growth with an approximately fourfold increase in doubling time and induced apoptosis in K562 cells. K562 cells were stably transfected with ERK1 or the dominant inhibitory mutant of ERK1 (ERK1-KR). We studied the role of ERK activation in the growth-promoting and anti-apoptotic effect of Ras and its involvement in hemin-induced nonterminal erythroid differentiation using the BCR-ABL-positive K562 cell line as a model. However, Ras-induced activation of the mitogen-activated protein kinase ERK has been suggested to play a critical role in either growth or differentiation in different model systems. I think I've got a sysex if you can't find it, but I've never tried it.Inhibition of signaling through Ras in BCR-ABL-positive pluripotent K562 cells leads to apoptosis and spontaneous differentiation. Honestly think the MCU emu is the shortest route the where you want to go, if you can find the stuff. I keep a spreadsheet to track the thousands of configured signals (not all of which have been assigned in Live) I can go on and on. Next we took the feature to the BCR 2000.
Our Channel Strip Upgrade takes things one step further. I realize I'm rambling, but I am the BCR+Live evangelist. When we first released our script for the Novation LaunchControl XL we were taken aback by the positive feedback for our extended parameter mode allowing up to 24 parameters to be controlled at the same time with the available encoders. Set another one to turn to scroll scenes and fire the scene when you push it - all 1 control/gesture. Also, it's so fucking logical and user-friendly to use those top encoders that both turn and push for the clip editing controls: 4 of them to turn and scroll region/loop start/stop where pushing the same ones will set those points to the cursor. Velocity drive/random across 8 MIDI beat tracks for my LP on one preset, Max Polybeat probabilities and velocities on another, bass input compressor amp/cab chain selector, Operator Osc A, Operator Osc B, Operator Master, Operator Filter, etc. Those controls stay mapped on every preset and there's 2 rows of knobs that are "open" on each preset so those become specialized for little jobs. Even time sigs and record quantize, which I made M4L devices for. If you hit CTRL+M in an empty set, everything shaded blue is mapped.
I literally have every global Live control mapped in my template set (and EVERY control of 1 instance of Operator). The imagined functionality is great as long as similar params across devices share the same knob, but if the threshold for the gate is a different knob than it is for the compressor, it is a motherfucker to memorize it all, long term. I sincerely doubt one would have an easy time memorizing all the spatial indices of up to 4 banks of params for every Live device it's a cognitive clusterfuck. I find my way requires a lot of critical thinking to get the right setup.
If you could find the old Mackie Emulation stuff for the BCR you may be able to go that route, or use it as a basis for your own approach. That is, I haven't found a way to make it navigate tracks/devices and automap past the first 8 CCs, although I don't think it should be that hard to do the latter. I love my BCR and couldn't imagine using any other controller with Live, but there's lots of static mappings. You could tweak the control scripts, perhaps.